Nano-sized nEF

Human-derived lactic acid bacteria with superior in absorption

Nano-sized lactic acid bacterium nEFR is the sterilized lactic acid bacteria which is derived from human (Enterococcus Faecalis). The aggregation of the lactic acid bacteria dispersed during the production of the sterilized lactic acid bacteria and the absorption rate of the lactic acid bacteria is improved by patented nano-processing method.

It contains 5 trillion bacteria per 1gram of product, from which we are able to intake high-quality and high-function with in a small amount of lactic acid bacteria powder.

Photo of nano-sized lactic acid bacterium

High concentrated powdered lactic bacteria is produced by the process for sterilization, concentration of only lactic acid bacteria and pulverization after cultivation of the lactic acid bacteria. But during pulverization process, cells of the lactic acid bacteria aggregates and become clumps. When dissolved in to water, it becomes a suspension containing various particle size of clamp of bacterial cells.

Nano-sized lactic acid bacteria is the lactic acid bacteria with which the particle diameter is less than 1 μm (micron) and which is dispersed individually when dissolved in water.

60% of the immune cells in the body are concentrated in small intestine. Nano-sized lactic acid bacteria easily pass through the immune system of Peyer’s patches in small intestine, it works on immunomodulatory function through gut immunity.

Peyer's patches works immune system

Main evidence of lactic acid bacterium nEF

NK cell activity stimulates by intake of nano-sized lactic acid bacterium nEF

Intake of lactic acid bacteria stimulates natural killer (NK) cell activity of spleen. Moreover intake of nano-sized lactic acid bacterium nEF confirmed the dose-dependent effects on NK cell activity.
Confirmed on NK cell activity by nEF

Confirmed on NK cell activity by nEF
Confirmed on NK cell activity by nEF






Prevention against infection of norovirus for mouse by intake of nano-sized lactic acid bacterium nEF

In order to verify how the difference in “dispersibility” at the process of producing sterilizes lactic acid bacteria affects the effectiveness, biological defense function was investigated by norovirus infection test by the mouse using “non-nano lactic acid bacteria” which was sterilized and dried after cultivation and “nano lactic acid bacteria” which was treated by dispersion process at production.

Test method:
Mice which infected to norovirus are divided into 3 groups (control (distilled water) group, non-nano sized lactic acid bacteria group, nano-sized lactic acid bacteria group) and forcedly orally administered daily for 21 days after infection.
Fecal viral amount and neutralizing antibody value in serum blood 3 weeks after infection were measured by plaque assay.

<Number of virus in the stool>

When the amount of excreted virus is decreased, the risk of infection is decreased accordingly. Nano-sized lactic acid bacteria administered group significantly suppress fecal virus growth at the early stage such as 2 days or 3 days after infection more than non-nano lactic acid bacteria administered group.
nEF decreases norovirus by half

<Neutralizing antibody value in serum blood after infection>

Nano-sized lactic acid bacteria group also showed higher neutralizing antibody values that quantified the function of eliminating the virus.
nEF increases antibody value
Nano-sized lactic acid bacteria superior in absorbability can be expected to improve the more rapid prevention effect by exerting the effect of the lactic acid bacteria sufficiently.

Advantage of Nano-sized lactic acid bacterium nEF®(Patented)

Economy: Large amount of lactic acid bacteria can be ingested at low dose and also cost performance is higher.
Reliability: Evidences are already available as afunctional food material. The number of bacteria in final product can be counted.
Quality: Heated lactic acid bacteria is superior heat resistance, with stable quality.
Preservability: Superior in preservability. (Avoid high temperature and humidity and keep in normal temperature)
Sanitation: No concern for contaminating the production line because it is a sterilized lactic acid bacteria.
Convenience: Heated lactic acid bacteria have almost no taste and odor, so it can be used for various products in a wide range of applications.
Functionality: Immunomodulator/intestinal regulation effect as a biogenics material.

Introduction of raw materials

Product specification etc.

Product name: Nano-sized lactic acid bacterium nEF
Strain name: Enterococcus Faecalis
Country of origin: Japan
Appearance: Pale yellowish-white powder
Solubility: Water-insoluble is disperse in Water
Grain size: Passes through a 1-mm screen
Genetically modified: No used
Storage temperature: Below 20°C
Shipping unit (packaging): 1kg


窶「Trademark registration: Nano-sized lactic acid bacterium nEF.
 (Holder of trademark right: Bio-Lab. Co. Ltd.)
窶「Patent registration No.46212418
  Th1 inducer and its method of producing

Please beware of imitation products (lactic acid bacteria as a raw material).

Corporate Profile

Company name IHM Inc.
Headquarter 2F,Ichigo Higashigotanda Bldg.,1-6-3 Higashigotanda Shinagawa-ku Tokyo 141-0022
Tel +81-3-4221-2207
Capital 98 million yen
Representative Shinji Irie, President
Business line Wholesale of pharmaceuticals, quasi-drugs, health food,
cosmetics, medical devices, etc. Sales of functional food materials,
Health food products planning and development
Number of employees 30
Partner banks Hokuriku Bank, MUFG Bank and Kiraboshi Bank
Major partners KOKANDO Co., Ltd.,
TOKIWA Pharmaceutical Co., Ltd.,
DAITO Pharmaceutical Co., Ltd,
Group sales company Hokkaido Medical Systems Inc.
Yamato Medical Inc.
Shutoken Medical Inc.
Chubu Medical Systems Inc.
Kansai Medical Systems Inc.
Nishinihon Medical Inc.
Kyushu Medical Inc.
Life Medical Inc.
Membership of Organization The Tokyo Chamber of Commerce and Industry